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Effect of Various Carbon Sources in the Biosynthesis of
Polyhydroxyalkanoates Using Mangrovibacter Plantisponsor Umtkb-3
Hannah Syahirah binti Rapi
Supervisor: Dr. Vigneswari A/P Sevakumaran
Bachelor of Science (Biological Sciences)
School of Fundamental Science
Synthetic plastics ends up polluting marine environment and accumulating the land
for thousands of years due to its non-degradability. Thus, polyhydroxyalkanoates
(PHA) can replace conventional plastics as they are completely degradable. Poly-3-
hydroxybutyrate [P(3HB)] are members of PHA family which can be biosynthesized by
bacteria. This study aims to study the effect of various carbon sources in the
biosynthesis of P(3HB) using Mangrovibacter plantisponsor UMTKB-3 and to enhance
the P(3HB) production. This was done by limiting the nitrogen supplied and increase
carbon sources by regulating the carbon-to-nitrogen (C/N) ration to 20 and 30 in 72
h of incubation. Carbon sources used were glucose, fructose, sucrose and glycerol.
The highest P(3HB) yield was recorded when M. plantisponsor utilized sucrose at C/N
ratio 20 which resulted 2.352% ± 0.0153 . While for C/N ratio 30, the highest P(3HB)
yield was 2.118% ± 0.0051 when bacteria was fed with glycerol as sole carbon source.
401 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018