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Production of Exopolysaccharides (EPS) By Bacillus Cereus from Marphysa
Moribidi in Shake Flask Fermentation at Different Acidity
Shahrul Zamir Bin Mohmad
Supervisor: Dr. Azila binti Adnan
Bachelor of Science (Biological Sciences)
School of Fundamental Science
Exopolysaccharides (EPS) or also known as bacterial extracellular polymeric substance
are usually used as texturizers, viscosifiers and syneresis-lowering agent in food
industry. Because of it highly potential, the study for production of EPS by Bacillus
cereus from Marphysa moribidii was conducted as limited research on this species of
polychaetes’ bacteria. Three objective for this study that is the effect of acidity on EPS
production, glucose consumption and cell growth by Bacillus cereus isolated from
Marphysa moribidii in shake flask fermentation. The pH used is pH 4.0, 5.0 and 6.0.
Three types of analysis method was used for sample analysis that is Total
Carbohydrates Analysis, Nelson-Somogyi method and spread plate technique to
achieve the objective. Although pH 5 use more percentage of sugar that is 26.3%, but
it show the higher EPS production that is 0.168g . So pH 5 was concluded as suitable
pH used for fermentation of Bacillus cereus from gut Marphysa moribidii to obtain
more EPS.
357 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018