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The Effect of Cryoprotectant and Different Concentration on Malaysian<br />

Mahseer (Tor tambroides) sperm<br />

Hanim Hafiza binti Junaidi<br />

Supervisor: Dr Ivan Koh Chong Chu<br />

Bachelor of Science in Agrotechnology (Aquaculture)<br />

School of Fisheries and Aquaculture Sciences<br />

Malaysian Mahseer (Tor tambroides) has high commercial value but is ecological<br />

threatened due to over exploitation. It is difficult for Mahseer to breed naturally in<br />

captive conditions. Hence, investigation for the best protocol for sperm<br />

cryopreservation of Mahseer sperm is needed. We investigated best composition for<br />

cryodiluent using three cryoprotectants (Dimethyl sulfoxide (DMSO), methanol<br />

(MeOH), dimethylacetamide (DMA)) at three concentrations (10, 15 and 20%). Sperm<br />

were diluted at ratio of 1:49 into cryodiluent (ASP + cryoprotectant) and cooled in<br />

liquid nitrogen (LN) vapour at 10 cm height from LN before immersion at -60℃.<br />

Toxicity was evaluated by diluting sperm in 10% DMSO and MeoH and evaluating<br />

motility at set intervals. The results showed no significant difference in post-thaw<br />

motility of all type and concentrations of cryoprotectant. Both 10% MeoH and DMSO<br />

were not toxic to sperm up to 1 hour. In conclusion, species-specific protocol for<br />

Mahseer was established.<br />

486 | UMT UNDERGRADUATE RESEARCH DAY 2018

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