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Preliminarily Screening on the Methanolic Crude Extracts from Bruguiera<br />

sp. and Antioxidant Activity<br />

Rasyidah Binti Abdul Rashid<br />

Supervisor: Dr. Noor Wini binti Mazlan<br />

Bachelor of Science (Analytical and Environmental Chemistry)<br />

School of Marine and Environmental Sciences<br />

Universiti Malaysia Terengganu<br />

Bruguiera sp. is a sub-tropical mangrove found in coastal area. The aim of this study<br />

was to preliminarily screen the secondary metabolites from different parts of Bruguiera<br />

cylindrica, B. gymnorrhiza and B. hainesii which have been collected in Setiu. The<br />

methanolic crude extracts were obtained using methanolic cold extraction. 3a-Ecoumaroyltaraxerol<br />

and N,N-dinitropiperazine were identified from the crude extracts<br />

of the leaves of B. gymnorrhiza and B. cylindrical, respectively. The highest percentage<br />

yield were the crude extracts from calyx (3.89%) and stem (0.69%) of B. gymnorrhiza.<br />

The most potent antioxidant using DPPH scavenging activity showed that the crude<br />

extracts from the calyx and twigs of B. gymnorrhiza with inhibition concentration at<br />

50% (IC50) value of 0.5 mg/mL, followed by the leaves (IC50 of 1.3 mg/mL). The<br />

secondary metabolites from Bruguiera sp. were identified by using 1D and 2D-nuclear<br />

magnetic resonance (NMR) coupled with multivariate analysis (SIMCA P+14).<br />

1970 | UMT UNDERGRADUATE RESEARCH DAY 2018

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