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The effect of carbon enrichment growth of Chlorococcum sp.
Muhamad Zuraidi Bin Zainon
Supervisor: Dr Eminour Muzalina Binti Mustafa
Bachelor of Science in Agrotechnology (Aquaculture)
School of Fisheries and Aquaculture Sciences
Chlorococcum sp. is one of the freshwater species that have high value of carotenoid
(astaxanthin) that usually used as feed in aquaculture industry. The objective of this
study is to assess the growth of Chlorococcum sp. in Bold’s Basal Medium(BBM) and
aquaculture wastewater enriched with different types of Carbon source (Glucose and
Acetate) for 12 days under autotrophic, heterotrophic & mixotrophic conditions. In
this study, there are 2 set of experiments were conducted. The Chlorococcum sp. were
grown in different types of medium which are BBM and aquaculture wastewater
enriched with glucose & acetate individually for 12 days under autotrophic,
heterotrophic & mixotrophic conditions. The growth of Chlorococcum sp. cultures were
measured based on chlorophyll a, carotenoid, optical density (OD620nm), and dry
weight content. The result showed that cultures grown in heterotrophic-BBM
containing glucose in dark condition have the highest Chl a content (1.560 mg/L Chl
a) and carotenoid content (672.4978 µg/mL carotenoid) was the highest in the
cultures grown in heterotrophic-BBM containing glucose in dark condition on day 8 .In
this study, we found that cultures grown in heterotrophic (BBM containing glucose) >
mixotrophic (BBM containing acetate) > mixotrophic (BBM containing glucose) >
heterotrophic (BBM containing acetate) > heterotrophic (BBM) > autotrophic (BBM).
555 | UMT UNDERGRADUATE RESEARCH DAY 2018