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Study of Phytochemical properties and Antioxidant Activity of
Holothuria nobilis
Nur Syuhada Binti Mohamad Nasir
Dr. Asnuzilawati Binti Asari
Bachelor of Science (Chemical Sciences)
School of Fundamental Science
Holothuria nobilis is one of the sea cucumber's species from the family of
Holothuriidae. It has been used as a traditional remedy by Malay and Chinese
community in Malaysia to treat asthma, hypertension and wound healing. In this
study, the sample of Holothuria nobilis were collected from Bidong Island, Terengganu
on 2016. The clean and dried samples of Holothuria nobilis were extracted using two
different solvent with different polarities which are ethyl acetate and methanol.
Phytochemical screening test was conducted on both crude extracts and each crude
shows positive results on flavonoid, saponin and glycoside which are responsible for
antioxidant properties. The dried sample and extract of Holothuria nobilis were
evaluate for their antioxidant activities such as full antioxidant assay using linoleic
acid, free radical scavenging assay using DPPH radical with superoxide radical and
non-enzymatic antioxidant assay which to determine a-tocopherol and carotenoid.
466 | U M T U N D E R G R A D U A T E R E S E A R C H D A Y 2018