Volumen II - SAM

- Las propiedades de sellado y ángulo de contacto de la película activa, características superficiales
importantes en la performance de un material factible de ser empleado en la fabricación de envases, no
mostraron diferencias significativas luego del tratamiento de activación.
- Se estudió la adsorción de lactocina 705 al PE y se determinó que ocurren, simultáneamente, procesos de
adsorción y descomposición parcial de la bacteriocina. Se optimizaron en 30ºC y 60 minutos la temperatura
y el tiempo de operación para la obtención de la película activa. Se obtuvieron las isotermas de adsorción que
responden a una ecuación tipo Langmuir.
En resumen y si bien nisina es la única bacteriocina aprobada al presente como aditivo alimentario, aquí
demostramos la eficacia de otras bacteriocinas para el desarrollo de nuevas estrategias de biopreservación.
5. REFERENCIAS
1. M. Blanco Massani, M. R. Fernandez; A. Ariosti; P. Eisenberg and G. Vignolo. “Development and
characterization of an active polyethylene film containing Lactobacillus curvatus CRL705
bacteriocins”. Food Additives and contaminants, Vol. 25, No. 11(2008) p. 1424–1430
2. T Pongtharangkul, A. Demirci “Evaluation of agar diffusion bioassay for nisin quantification”. Applied
Microbiology and Biotechnology, 65 (2004) p. 268–272.
3. ASTM F 88-06 “Standard test method for seal strength of flexible barrier materials.” Philadelphia, PA:
American Society for Testing and Materials.
4. Resolución GMC N° 36/92 del MERCOSUR “Ensayos de migración total de embalajes y
equipamientos plásticos en contacto con alimentos”.
5. J. Palacios, G. Vignolo, M. E. Farias, A. Pesce de Ruiz Holgado, G. Oliver, and F. Sesma..
“Purification and amino acid sequence of lactocin 705, a bacteriocin produced by Lactobacillus casei
CRL 705.” Microbiology Research, 154 (1999) p. 199–204.
6. S. A.Cuozzo, F Sesma, J.M. Palacios, A. Pesce de Ruiz Holgado, and R.R. Raya. “Identification and
nucleotide sequience of genes involved in the synthesis of lactocin 705, a two-peptide bacteriocin from
Lactobacillus casei CRL 705.” FEMS Microliology Letters, 185 (2000) p. 157-161.
7. S. A.Cuozzo, P. Castellano, J. M Sesma, G. Vignolo, R.R .Raya “Differential Roles of the Two-
Component Peptides of Lactocin 705 in Antimicrobial Activity.” Current Microbiology, 46 (2003) p.
180-183.
8. P. Roach, D. Farrar, and C.C. Perry “Interpretation of protein adsorption: Surface-induced
conformational changes.” Journal of the American Chemical Society, 127 (2005) p. 8168-8173
9. J Neil Shirtcliffe, A Sanaa, C Evans, G McHale, M I Newton, C C Perry and P Roach “The use of high
aspect ratio photoresist (SU-8) for super-hydrophobic pattern prototyping.” Journal of micromechanics
and microengineering. 14(2004) p.1384-1389
10. S.Guruvenket, G.Mohan Rao, M Komath and A.M.Raichur. “Plasma surface modification of
polystyrene and polyethylene.” Applied Surface Science 236 (2004) p.278–284
11. M. Ataeefard, S Moradiana, , M Mirabedinib, M Ebrahimia and S Asiabanc “Investigating the effect of
power/time in the wettability of Ar and O2 gas plasma-treated low-density polyethylene” Progress in
Organic Coatings, 64 (2009) p. 482–488
12. M F Porto, E M Girotto, M H Kunita, M do Carmo Gonçalves, E C Muniz, A F Rubira, E Radovanovic
“Atomic force microscopy, scanning electric potential microscopy and contact-angle surface analysis of
low-density polyethylene grafted with maleic anhydride.” Progress in Colloid and Polymer Science,
128 (2004) p. 86–91
13. MA Daeschel, JM McGuire, H. Al-Makhlafi “Antimicrobial activity of nisin adsorbed to hydrophilic
and hydrophobic silicon surfaces.” Journal of Food Protection, Vol 55 (1992). No 9 p. 731–735.
14. Ll. Burianek, and AE Yousef. “Solvent extraction of bacteriocins from liquid cultures.” Letters in
applied microbiology, 31 (2000) p. 193-197
15. P. Castellano, G. Vignolo, RN. Farías, JL. Arrondo, and R. Cheín “Molecular view by fourier transform
infrared spectroscopy of the relationship between lactocin 705 and membranes: speculations on
antimicrobial mechanism.” Applied and Environmental Microbiology, Vol 73, (2007) No 2p. 415-420
16. W. Norde, F. Macritchie, G. Nowicka and J. Lyklema “Protein adsorption at solid-liquid interfaces:
reversibility and conformation aspects.” Journal of Colloid and Interface Science, Vol. 112 (1986) No2
p. 447-456
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